active+pro Caspase 3 (23C9) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52439R-100uL |
| Product Name | active+pro Caspase 3 (23C9) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein |
| Clonality | Monoclonal |
| Clone # | 23C9 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 836 |
| Swiss Prot | P42574 |
| Synonyms | Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP-3, CPP-32, SCA-1, CASP3 |
| Background | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is active+pro Caspase 3 (23C9) Monoclonal Antibody (bsm-52439R) validated for, and at what dilution?
bsm-52439R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does active+pro Caspase 3 (23C9) Monoclonal Antibody (bsm-52439R) react with?
Tested reactivity: Human.
How should bsm-52439R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise active+pro Caspase 3 (23C9) Monoclonal Antibody (bsm-52439R)?
Recombinant protein