Caspase 1 (1E8) Monoclonal Antibody

Flow cytometric analysis of Hela cells with Caspase-1 (1E8 ) Monoclonal Antibody (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

$Lane 1: THP-1 cells; Lane 2: Jurkat cell lysate probed with Caspase-1 (1E8 ) Monoclonal Antibody (bsm-52441R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: THP-1 cells; Lane 2: Jurkat cell lysate probed with Caspase-1 (1E8 ) Monoclonal Antibody (bsm-52441R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Caspase-1 (1E8) Monoclonal Antibody (bsm-52441R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Caspase-1 (1E8) Monoclonal Antibody (bsm-52441R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Caspase-1 (1E8) Monoclonal Antibody (bsm-52441R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Caspase-1 (1E8) Monoclonal Antibody (bsm-52441R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52441R
Product Name	Caspase 1 (1E8) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal Human Caspase-1
Clonality	Monoclonal
Clone #	1E8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	834
Swiss Prot	P29466
Synonyms	Caspase-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, CASP-1, IL-1BC, ICE, IL-1 beta-converting enzyme, CASP1
Background	Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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