PKC beta 2 (10A1) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52464R-100uL |
| Product Name | PKC beta 2 (10A1) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human PKC beta 2 between 600 to C-terminus |
| Clonality | Monoclonal |
| Clone # | 10A1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5579 |
| Swiss Prot | P05771 |
| Synonyms | Protein kinase C beta type, PKC-B, PKC-beta, PRKCB, PKCB, PRKCB1 |
| Background | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional PKC isoforms (_, _I, _II and _) and novel PKC isoforms. Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC _ and are independent of Ca2+. On the other hand, nPKC _ and _, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is PKC beta 2 (10A1) Monoclonal Antibody (bsm-52464R) validated for, and at what dilution?
bsm-52464R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKC beta 2 (10A1) Monoclonal Antibody (bsm-52464R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52464R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKC beta 2 (10A1) Monoclonal Antibody (bsm-52464R)?
Human PKC beta 2 between 600 to C-terminus