nNOS Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with nNOS monoclonal antibody, unconjugated (bsm-52474R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with nNOS monoclonal antibody, unconjugated (bsm-52474R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Predicted Reactivity

Human
Mouse

Overview
Catalog #	bsm-52474r
Product Name	nNOS Recombinant Antibody
Applications	WB
Reactivity	Rat
Predicted Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminus human nNOS
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4842
Swiss Prot	P29475
Synonyms	Nitric oxide synthase, brain, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, bNOS, N-NOS, nNOS, NOS1
Background	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Application Dilution
WB	1:500-1000