Collagen I Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52478r-bf405-100uL |
| Product Name | Collagen I Recombinant Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Cow |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | Synthetic peptide of human Collagen I between 1200 to 1300 amino acids |
| Immunogen Range | 1191-1238/1464 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1277 |
| Swiss Prot | P02452 |
| Subcellular location | Extracellular |
| Synonyms | Collagen alpha-1(I) chain; Alpha-1 type I collagen; Collagen alpha-2(I) chain; Alpha-2 type I collagen; COL1A1; COL1A2. |
| Background | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
| Application Dilution | |
| WB | 1:200-400 |
| FCM | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Collagen I Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-52478R-BF405) validated for, and at what dilution?
bsm-52478R-BF405 is validated for WB (1:200-400); FCM (1:100-500); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Collagen I Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-52478R-BF405) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow.
How should bsm-52478R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Collagen I Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-52478R-BF405)?
Synthetic peptide of human Collagen I between 1200 to 1300 amino acids Immunogen range: 1191-1238/1464.
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-52478R-BF405)?
AbBy Fluor-405 on bsm-52478R-BF405: excitation / emission 401nm/421nm.