Pan-Cadherin Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52480R-100uL |
| Product Name | Pan-Cadherin Recombinant Antibody |
| Applications |
WB
IF(ICC)
IHC
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | C-terminus human Cadherin |
| Clonality | Monoclonal |
| Clone # | 9A1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Swiss Prot | P12830; P19022; P22223 |
| Synonyms | Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, CD_antigen: CD324, Cadherin-2, CDw325, Neural cadherin, CD_antigen: CD325, Cadherin-3, Placental cadherin, 7B4 antigen, Vascular endothelial cadherin, CD_antigen: CD144, Cadherin-4, Retinal cadherin, E-cadherin, CDH1, CDHE, UVO, CDH2, CDHN, NCAD, P-cadherin, CDH3, CDHP, VE-cadherin, CDH5, R-CAD, R-cadherin |
| Background | Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin, R-cadherin, B-cadherin, E/P cadherin and cadherin-5. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Pan-Cadherin Recombinant Antibody (bsm-52480R) validated for, and at what dilution?
bsm-52480R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Pan-Cadherin Recombinant Antibody (bsm-52480R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52480R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Pan-Cadherin Recombinant Antibody (bsm-52480R)?
C-terminus human Cadherin