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S100B Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52506r-cy3-100uL
Product Name S100B Recombinant Antibody, Cy3 Conjugated
Applications
WB IF FLOW-CYT
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source A synthesized peptide derived from human S100B
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6285
Swiss Prot P04271
Subcellular location Cytoplasm, Nucleus
Synonyms NEF; S100; S100-B; S100beta; S100B_HUMAN; S100B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100 calcium binding protein B; S100 calcium binding protein, beta (neural)
Application Dilution
WB 1:1000-2000
IF 1:200-800
Flow-Cyt 1:100-200

Frequently asked questions

Which applications is S100B Recombinant Antibody, Cy3 Conjugated (bsm-52506R-Cy3) validated for, and at what dilution?

bsm-52506R-Cy3 is validated for WB (1:1000-2000); IF (1:200-800); Flow-Cyt (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does S100B Recombinant Antibody, Cy3 Conjugated (bsm-52506R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-52506R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise S100B Recombinant Antibody, Cy3 Conjugated (bsm-52506R-Cy3)?

A synthesized peptide derived from human S100B

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-52506R-Cy3)?

Cy3 on bsm-52506R-Cy3: excitation / emission 512,550nm/570,615nm.