HNRNPK (8G5) Monoclonal Antibody

$Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: NIH\/3T3 Cells; Probed with HNRNPK (8G5) Monoclonal Antibody (bsm-52510RR) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: NIH\/3T3 Cells; Probed with HNRNPK (8G5) Monoclonal Antibody (bsm-52510RR) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52510R
Product Name	HNRNPK (8G5) Monoclonal Antibody
Applications	WB IHC-P IF(ICC) IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	N terminus Human HNRNPK
Clonality	Monoclonal
Clone #	8G5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	3190
Swiss Prot	P61978
Synonyms	Heterogeneous nuclear ribonucleoprotein K, Transformation up-regulated nuclear protein, hnRNP K, TUNP, HNRNPK
Background	One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC