PRP19 (7H10) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52524R-100uL |
| Product Name | PRP19 (7H10) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human PRP19 between 450-550 amino acids |
| Clonality | Monoclonal |
| Clone # | 7H10 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 27339 |
| Swiss Prot | Q9UMS4 |
| Synonyms | Pre-mRNA-processing factor 19, Nuclear matrix protein 200, PRP19/PSO4 homolog, Senescence evasion factor, hPso4, PRPF19 |
| Background | Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair (PubMed:17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response (PubMed:24332808). May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA (PubMed:18263876). As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process (PubMed:16223718). In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:11435423). May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is PRP19 (7H10) Monoclonal Antibody (bsm-52524R) validated for, and at what dilution?
bsm-52524R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PRP19 (7H10) Monoclonal Antibody (bsm-52524R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52524R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PRP19 (7H10) Monoclonal Antibody (bsm-52524R)?
Human PRP19 between 450-550 amino acids