SMC1 (Ser957) (8H5) Monoclonal Antibody

$Hela cell lysate probed with SMC1 (Ser957) (8H5) Monoclonal Antibody (bsm-52525R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Hela cell lysate probed with SMC1 (Ser957) (8H5) Monoclonal Antibody (bsm-52525R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Phospho-SMC1 (S957) (8H5) Monoclonal Antibody (bsm-52525R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Phospho-SMC1 (S957) (8H5) Monoclonal Antibody (bsm-52525R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with Phospho-SMC1 (S957) (8H5) Monoclonal Antibody (bsm-52525R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with Phospho-SMC1 (S957) (8H5) Monoclonal Antibody (bsm-52525R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Phospho-SMC1 (S957) (8H5) Monoclonal Antibody (bsm-52525R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Phospho-SMC1 (S957) (8H5) Monoclonal Antibody (bsm-52525R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human

Overview
Catalog #	bsm-52525R
Product Name	SMC1 (Ser957) (8H5) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to residues surrounding Ser957 of human SMC1
Modification Site	Ser957
Clonality	Monoclonal
Clone #	8H5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	8243
Swiss Prot	Q14683
Synonyms	Structural maintenance of chromosomes protein 1A, Sb1.8, Structural maintenance of chromosomes protein 1B, SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1A, SMC protein 1B, SMC-1-beta, SMC-1B, SMC1B
Background	Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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