Sonic Hedgehog Protein (C-Product) (4C10) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52529R |
| Product Name | Sonic Hedgehog Protein (C-Product) (4C10) Monoclonal Antibody |
| Applications |
WB
FCM
IHC-P
IF(ICC)
IHC
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human Sonic Hedgehog between 250-350 amino acids |
| Clonality | Monoclonal |
| Clone # | 4C10 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 6469 |
| Swiss Prot | Q15465 |
| Synonyms | Sonic hedgehog protein, HHG-1, SHH |
| Background | Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IF(ICC) | 1:50-200 |
| IHC | |
Frequently asked questions
Which applications is Sonic Hedgehog Protein (C-Product) (4C10) Monoclonal Antibody (bsm-52529R) validated for, and at what dilution?
bsm-52529R is validated for WB (1:300-5000); FCM (1:20-100); IHC-P (1:200-400); IF(ICC) (1:50-200); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Sonic Hedgehog Protein (C-Product) (4C10) Monoclonal Antibody (bsm-52529R) react with?
Tested reactivity: Human.
How should bsm-52529R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Sonic Hedgehog Protein (C-Product) (4C10) Monoclonal Antibody (bsm-52529R)?
Human Sonic Hedgehog between 250-350 amino acids