Mast Cell Tryptase Recombinant Antibody

Name: Mast Cell Tryptase Recombinant Antibody
Brand: Bioss
SKU: bsm-52533R
Price: 490.0 USD
Availability: InStock

$Paraformaldehyde-fixed, paraffin embedded (human Abdominal skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human Abdominal skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (Mouse Stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Mouse Stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (Mouse Skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Mouse Skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$25 ug total protein per lane of various lysates (see on figure) probed with Mast Cell Tryptase monoclonal antibody, unconjugated (bsm-52533R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Mast Cell Tryptase monoclonal antibody, unconjugated (bsm-52533R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mast Cell Tryptase Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mast Cell Tryptase Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mast Cell Tryptase Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mast Cell Tryptase Antibody, Unconjugated (bsm-52533R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52533r-100uL
Product Name	Mast Cell Tryptase Recombinant Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human Tryptase 1
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7177
Swiss Prot	Q15661
Subcellular location	Secreted
Synonyms	Tryptase delta; delta-tryptase; HmMCP-3-like tryptase III; Mast cell mMCP-7-like; Tryptase-3; TPSD1; MCP7L1; MMCP-7L; MCP7-LIKE; EC 3.4.21.59; MGC95428; TRYD; Tryptase3; alpha II; EC 3.4.21.59; Lung tryptase; Mast cell alpha II tryptase; Mast cell beta I tryptase; Mast cell protease 7; Mast cell protease II; MCP 7; MMCP 7; Pituitary tryptase; Skin tryptase; TPS 1; TPS1; TPS2; TPSAB1; TPSAB1 protein; TPSB1; Tryptase 1; Tryptase alpha 1; tryptase alpha I included; Tryptase alpha II; tryptase alpha II included; tryptase alpha included; tryptase alpha/beta 1; Tryptase beta 1; tryptase beta I included; Tryptase I; tryptase I included; Tryptase III; Tryptase skin; Tryptase beta-2; TRYB1_HUMAN; TRYB2_HUMAN; TRYD_HUMAN.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF

Frequently asked questions

Which applications is Mast Cell Tryptase Recombinant Antibody (bsm-52533R) validated for, and at what dilution?

bsm-52533R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Mast Cell Tryptase Recombinant Antibody (bsm-52533R) react with?

Tested reactivity: Human, Mouse.

How should bsm-52533R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Mast Cell Tryptase Recombinant Antibody (bsm-52533R)?

A synthesized peptide derived from human Tryptase 1

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Mast Cell Tryptase Recombinant Antibody

Applications

Conjugation Options

Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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