Caspase-9 Recombinant Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52566r-apc-cy7 |
| Product Name | Caspase-9 Recombinant Antibody, APC-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | Human Caspase-9 aa 200-350 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P55211 |
| Synonyms | Caspase-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, CASP-9, APAF-3, ICE-LAP6, CASP9 |
| Background | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Caspase-9 Recombinant Antibody, APC-Cy7 Conjugated (bsm-52566R-APC-Cy7) validated for, and at what dilution?
bsm-52566R-APC-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase-9 Recombinant Antibody, APC-Cy7 Conjugated (bsm-52566R-APC-Cy7) react with?
Tested reactivity: Human, Mouse.
How should bsm-52566R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caspase-9 Recombinant Antibody, APC-Cy7 Conjugated (bsm-52566R-APC-Cy7)?
Human Caspase-9 aa 200-350
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bsm-52566R-APC-Cy7)?
APC-Cy7 on bsm-52566R-APC-Cy7: excitation / emission 650nm/780nm.