Hes1 Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52568r-bf405 |
| Product Name | Hes1 Recombinant Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human HES1 : 200-280/280 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3280 |
| Swiss Prot | Q14469 |
| Synonyms | Transcription factor HES-1, Class B basic helix-loop-helix protein 39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, bHLHb39, hHL, HES1 |
| Background | Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Hes1 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-52568R-BF405) validated for, and at what dilution?
bsm-52568R-BF405 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Hes1 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-52568R-BF405) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52568R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hes1 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-52568R-BF405)?
A synthesized peptide derived from human HES1 : 200-280/280
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-52568R-BF405)?
AbBy Fluor-405 on bsm-52568R-BF405: excitation / emission 401nm/421nm.