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Tyrosine Hydroxylase Recombinant Antibody, PerCP-Cy5.5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52574r-percp-Cy5.5-100uL
Product Name Tyrosine Hydroxylase Recombinant Antibody, PerCP-Cy5.5 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation PerCP-Cy5.5
Host Rabbit
Source Human Tyrosine Hydroxylase between 500 amino acid to C-terminus
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7054
Swiss Prot P07101
Synonyms Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH; TYH
Background Neuronal Marker Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.
Application Dilution
WB IFICC1:50-200
FCM WB1:500-2000
IF(IHC-P) IHC-P1:100-500
IF(ICC) FCM1:50-100

Frequently asked questions

Which applications is Tyrosine Hydroxylase Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-52574R-PERCP-CY5-5) validated for, and at what dilution?

BSM-52574R-PERCP-CY5-5 is validated for WB(IF(ICC)) (1:50-200); FCM(WB) (1:500-2000); IF(IHC-P)(IHC-P) (1:100-500); IF(ICC)(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Tyrosine Hydroxylase Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-52574R-PERCP-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat.

How should BSM-52574R-PERCP-CY5-5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Tyrosine Hydroxylase Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-52574R-PERCP-CY5-5)?

Human Tyrosine Hydroxylase between 500 amino acid to C-terminus

What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BSM-52574R-PERCP-CY5-5)?

PerCP-Cy5.5 on BSM-52574R-PERCP-CY5-5: excitation / emission 482nm/694nm.