MUC1 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52576r-rbitc-100uL |
| Product Name | MUC1 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | Human MUC1 between 1-100 amino acids |
| Clonality | Monoclonal |
| Clone # | 5G5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4582 |
| Swiss Prot | P15941 |
| Synonyms | Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CD_antigen: CD227, MUC-1, CA 15-3, KL-6, PUM, PEM, EMA, MUC1 |
| Background | The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity. |
| Application Dilution | |
| WB | 1:1000-5000 |
| IF(IHC-P) | IFIHC-P1:200-800 |
| IF(ICC) | IFICC1:100-200 |
Frequently asked questions
Which applications is MUC1 Recombinant Antibody, RBITC Conjugated (bsm-52576R-RBITC) validated for, and at what dilution?
bsm-52576R-RBITC is validated for WB(WB) (1:1000-5000); IF(IHC-P)(IF(IHC-P)) (1:200-800); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MUC1 Recombinant Antibody, RBITC Conjugated (bsm-52576R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52576R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MUC1 Recombinant Antibody, RBITC Conjugated (bsm-52576R-RBITC)?
Human MUC1 between 1-100 amino acids
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-52576R-RBITC)?
RBITC on bsm-52576R-RBITC: excitation / emission 570nm/595nm.