PP2A alpha + beta (11F10) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52611R-100uL |
| Product Name | PP2A alpha + beta (11F10) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
IHC-P
|
| Reactivity | Human, Mouse, Rat, Zebrafish |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human PP2A alpha + beta aa 250-350 |
| Clonality | Monoclonal |
| Clone # | 11F10 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Swiss Prot | P62714, P67775 |
| Synonyms | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2A-alpha, RP-C, PPP2CA, PP2A-beta, PPP2CB |
| Background | The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) validated for, and at what dilution?
bsm-52611R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) react with?
Tested reactivity: Human, Mouse, Rat, Zebrafish.
How should bsm-52611R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R)?
Human PP2A alpha + beta aa 250-350