Glycogen synthase (1A8) Monoclonal Antibody

Flow cytometric analysis of 293 cells with Glycogen synthase (1A8) Monoclonal Antibody (bsm-52615R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Hela cells probed with Glycogen synthase (1A8) Monoclonal Antibody (bsm-52615R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Hela cells probed with Glycogen synthase (1A8) Monoclonal Antibody (bsm-52615R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52615R
Product Name	Glycogen synthase (1A8) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Glycogen synthase between 700 to 800 amino acids
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	2997
Swiss Prot	P13807
Synonyms	Glycogen [starch] synthase, muscle, GYS1
Background	Glycogen Synthase (GS) is a key enzyme in the regulation of glycogen metabolism. GS catalyzes the incorporation of UDP-glucose incorporation into glycogen. The activity of glycogen synthase is regulated by hormonal stimuli (insulin, catecholamines and glucagons) and non-hormonal stimuli (blood glucose level and exercise). Two main isoforms of mammalian GS are designated as muscle (glycogen synthase 1) and liver (glycogen synthase 2). Most tissues express glycogen synthase 1, whereas glycogen synthase 2 appears to be tissue-specific. The two isoforms have 70% identical amino acid sequence. Glycogen synthase can be phosphorylated by multiple kinases including glycogen synthase kinase-3 (GSK-3), mitogen-activated protein kinase-related protein kinase (DYRK), and SAPK2b/p38b which leads to its inactivation.
Application Dilution
WB	1:300-5000
FCM	1:20-100