Stromal interaction molecule 1 (7C4) Monoclonal Antibody

$Lane 1: K562 Cells; Lane 2:HepG2 Cells; Probed with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cells; Lane 2:HepG2 Cells; Probed with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52677R
Product Name	Stromal interaction molecule 1 (7C4) Monoclonal Antibody
Applications	WB IHC-P IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Stromal interaction molecule 1 between 1-600 amino acids
Clonality	Monoclonal
Clone #	7C4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	6786
Swiss Prot	Q13586
Synonyms	Stromal interaction molecule 1, STIM1
Background	Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC