PAX6 (8T3) Monoclonal Antibody

Flow cytometric analysis of sh-sy5y cells with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Mouse eye lysate probed with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Mouse eye lysate probed with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat eyes tissue incubated with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat eyes tissue incubated with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse eyes tissue incubated with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse eyes tissue incubated with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52682R
Product Name	PAX6 (8T3) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human PAX6 between 250 to 350 amino acids
Immunogen Range	250-350/422
Clonality	Monoclonal
Clone #	8T3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P26367
Synonyms	Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6
Background	Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC

Applications

Reactivity

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