PAX6 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52682r-cy3-100uL |
| Product Name | PAX6 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Human PAX6 between 250 to 350 amino acids |
| Immunogen Range | 250-350/422 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P26367 |
| Synonyms | Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6 |
| Background | Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes. |
| Application Dilution | |
| WB | 1:500-3000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is PAX6 Recombinant Antibody, Cy3 Conjugated (bsm-52682R-Cy3) validated for, and at what dilution?
bsm-52682R-Cy3 is validated for WB(WB) (1:500-3000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PAX6 Recombinant Antibody, Cy3 Conjugated (bsm-52682R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52682R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PAX6 Recombinant Antibody, Cy3 Conjugated (bsm-52682R-Cy3)?
Human PAX6 between 250 to 350 amino acids Immunogen range: 250-350/422.
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-52682R-Cy3)?
Cy3 on bsm-52682R-Cy3: excitation / emission 512,550nm/570,615nm.