Cdc6 (Ser54) (6F5) Monoclonal Antibody

$Jurkat cells Probed with Phospho-Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Jurkat cells Probed with Phospho-Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-52708R
Product Name	Cdc6 (Ser54) (6F5) Monoclonal Antibody
Applications	WB IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide surrounding Ser54 of human Cdc6
Modification Site	Ser54
Clonality	Monoclonal
Clone #	6F5
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	990
Swiss Prot	Q99741
Synonyms	Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, p62(cdc6), HsCdc18, HsCDC6, CDC6
Background	Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200