MEKK3 (5C1) Monoclonal Antibody

Flow cytometric analysis of HeLa cells with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: A431 Cells; Probed with MEKK3 (5C1) Monoclonal Antibody (bsm-54089R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: A431 Cells; Probed with MEKK3 (5C1) Monoclonal Antibody (bsm-54089R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:300 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with MEKK3 (5C1) Monoclonal Antibody (bsm-52709R) at 1:300 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse uterus tissue incubated with MEKK3 (5C1) (6F5) Monoclonal Antibody (bsm-52709R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse uterus tissue incubated with MEKK3 (5C1) (6F5) Monoclonal Antibody (bsm-52709R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52709R
Product Name	MEKK3 (5C1) Monoclonal Antibody
Applications	WB, FCM, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human MEKK3 between 300 to 600 amino acids
Clonality	Monoclonal
Clone #	5C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4215
Swiss Prot	Q99759
Synonyms	Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3, MAP3K3
Background	Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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