TSG101 Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52746r-bf350-100uL |
| Product Name | TSG101 Recombinant Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | Human TSG101 between 1 to 100 amino acids |
| Immunogen Range | 30-70/390 |
| Clonality | Monoclonal |
| Clone # | 6C3 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7251 |
| Swiss Prot | Q99816 |
| Synonyms | Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101 |
| Background | Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is TSG101 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52746R-BF350) validated for, and at what dilution?
bsm-52746R-BF350 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSG101 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52746R-BF350) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52746R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSG101 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52746R-BF350)?
Human TSG101 between 1 to 100 amino acids Immunogen range: 30-70/390.
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-52746R-BF350)?
AbBy Fluor-350 on bsm-52746R-BF350: excitation / emission 346nm/442nm.