SUZ12 (3C1) Monoclonal Antibody

$MCF-7 cell lysate probed with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

MCF-7 cell lysate probed with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Bone lysates; Lane 3: Mouse Uterus lysates; Lane 4: RAT Liver cell lysates; Lane 5: Human JAR cell lysates; Lane 6: Human MDA-MB-231 cell lysates; Lane 7: Human SH-SY5Y cell lysates probed with SUZ12 (3C1) Monoclonal Antibody, Unconjugated (bsm-52749R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52749R
Product Name	SUZ12 (3C1) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human SUZ12 between 50 to 150 amino acids
Clonality	Monoclonal
Clone #	3C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	23512
Swiss Prot	Q15022
Synonyms	Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, ChET 9 protein, SUZ12
Background	Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200

Applications

Reactivity

Search our catalog of hundreds of thousands of products