Laminnin 5 alpha 3 (8C1) Monoclonal Antibody

$A431 cells Probed with Laminnin 5 alpha 3 (8C1) Monoclonal Antibody (bsm-52787R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

A431 cells Probed with Laminnin 5 alpha 3 (8C1) Monoclonal Antibody (bsm-52787R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Laminnin 5 alpha 3 (8C1) Monoclonal Antibody (bsm-52787R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Laminnin 5 alpha 3 (8C1) Monoclonal Antibody (bsm-52787R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Laminnin 5 alpha 3 (8C1) Monoclonal Antibody (bsm-52787R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Laminnin 5 alpha 3 (8C1) Monoclonal Antibody (bsm-52787R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-52787R
Product Name	Laminnin 5 alpha 3 (8C1) Monoclonal Antibody
Applications	WB IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Laminnin 5 alpha 3 aa 1600-1800
Clonality	Monoclonal
Clone #	8C1
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3909
Swiss Prot	Q16787
Synonyms	Laminin subunit alpha-3, Epiligrin 170 kDa subunit, Epiligrin subunit alpha, Kalinin subunit alpha, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, Nicein subunit alpha, E170, LAMA3
Background	Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200