TFRC Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52793r-bf647-100uL |
| Product Name | TFRC Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | Human Transferrin Receptor between 100 to 450 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | P02786 |
| Swiss Prot | P02786 |
| Synonyms | Transferrin receptor protein 1, T9, p90, CD_antigen: CD71, TR, TfR, TfR1, Trfr, TFRC |
| Background | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is TFRC Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52793R-BF647) validated for, and at what dilution?
bsm-52793R-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TFRC Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52793R-BF647) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-52793R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TFRC Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52793R-BF647)?
Human Transferrin Receptor between 100 to 450 amino acids
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-52793R-BF647)?
AbBy Fluor-647 on bsm-52793R-BF647: excitation / emission 650nm/665nm.