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Thymidine Kinase 1 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with Thymidine kinase 1 monoclonal antibody, unconjugated (bsm-52813R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Thymidine kinase 1 monoclonal antibody, unconjugated (bsm-52813R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The U-2 OS (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Thymidine kinase 1 antibody (bsm-52813R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The U-2 OS (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Thymidine kinase 1 antibody (bsm-52813R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IP
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-52813r
Product Name Thymidine Kinase 1 Recombinant Antibody
Applications
WB IHC-P IP FLOW-CYT ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Human Thymidine Kinase 1 around C terminus
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 7083
Swiss Prot P04183
Synonyms Thymidine kinase, cytosolic, TK1
Background Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IP 1-2ug
Flow-Cyt 1ug/Test
ICC/IF 1:50-100

Frequently asked questions

Which applications is Thymidine Kinase 1 Recombinant Antibody (bsm-52813R) validated for, and at what dilution?

bsm-52813R is validated for WB (1:300-5000); IHC-P (1:200-400); IP (1-2ug); Flow-Cyt (1ug/Test); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Thymidine Kinase 1 Recombinant Antibody (bsm-52813R) react with?

Tested reactivity: Human.

How should bsm-52813R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Thymidine Kinase 1 Recombinant Antibody (bsm-52813R)?

Human Thymidine Kinase 1 around C terminus

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