Thymidine Kinase 1 Recombinant Antibody

Flow cytometric analysis of HeLa cells with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: 293T lysates; Lane 2: Hela lysates; Probed with Thymidine Kinase 1 (9C1)Monoclonal Antibody (bsm-52813R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293T lysates; Lane 2: Hela lysates; Probed with Thymidine Kinase 1 (9C1)Monoclonal Antibody (bsm-52813R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:100 in NIH\/3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:100 in NIH\/3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IP
Flow-Cyt
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-52813r
Product Name	Thymidine Kinase 1 Recombinant Antibody
Applications	WB, IHC-P, IP, Flow-Cyt, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Thymidine Kinase 1 around C terminus
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7083
Swiss Prot	P04183
Synonyms	Thymidine kinase, cytosolic, TK1
Background	Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IP	1-2ug
Flow-Cyt	1ug/Test
ICC/IF	1:50-100