Cardiac Troponin I (4C2) Monoclonal Antibody

$Human heart lysates probed with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Human heart lysates probed with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human heart tissue incubated with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human heart tissue incubated with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52818R
Product Name	Cardiac Troponin I (4C2) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Cardiac Troponin I between 1 to 150 amino acids
Clonality	Monoclonal
Clone #	4C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7137
Swiss Prot	P19429
Synonyms	Troponin I, cardiac muscle, Cardiac troponin I, TNNI3
Background	Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC