NQO1 Recombinant Antibody

Flow cytometric analysis of Hela cells with NQO1 (8T5) Monoclonal Antibody (bsm-52830R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: SH-SY-5Y lysates; Lane 2: Mouse kidney lysates; Line 3: Raji lysates ;Probed with NQO1 (8T5) Monoclonal Antibody (bsm-52830R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SH-SY-5Y lysates; Lane 2: Mouse kidney lysates; Line 3: Raji lysates ;Probed with NQO1 (8T5) Monoclonal Antibody (bsm-52830R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$25 ug total protein per lane of various lysates (see on figure) probed with NQO1 monoclonal antibody, unconjugated (bsm-52830R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with NQO1 monoclonal antibody, unconjugated (bsm-52830R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52830r-100ul
Product Name	NQO1 Recombinant Antibody
Applications	WB, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length protein
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	1728
Swiss Prot	P15559
Synonyms	NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1, NQO1
Background	The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Application Dilution
WB	1:300-5000
Flow-Cyt	1ug/Test
ICC/IF	1:50-100