PDI (6C9) Monoclonal Antibody

Flow cytometric analysis of HeLa cells with PDI (6C9) Monoclonal Antibody (bsm-52849R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: NIH\/3T3 lysates; Lane 2: Human liver lysates; Probed with PDI (6C9)Monoclonal Antibody (bsm-52849R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: NIH\/3T3 lysates; Lane 2: Human liver lysates; Probed with PDI (6C9)Monoclonal Antibody (bsm-52849R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52849R
Product Name	PDI (6C9) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human PD1 between 1 to 200 amino acids
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	5034
Swiss Prot	P07237
Synonyms	Protein disulfide-isomerase A2, PDIp, Pdia2
Background	This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).
Application Dilution
WB	1:300-5000
FCM	1:20-100