Neurofilament heavy polypeptide (4C3) Monoclonal Antibody

$Lane 1: mouse brain lysates; Probed with Neurofilament heavy polypeptide (4C3) Monoclonal Antibody (bsm-52851R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: mouse brain lysates; Probed with Neurofilament heavy polypeptide (4C3) Monoclonal Antibody (bsm-52851R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat cerebellumy tissue incubated with Neurofilament heavy polypeptide (4C3) Monoclonal Antibody (bsm-52851R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat cerebellumy tissue incubated with Neurofilament heavy polypeptide (4C3) Monoclonal Antibody (bsm-52851R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52851R
Product Name	Neurofilament heavy polypeptide (4C3) Monoclonal Antibody
Applications	WB, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Neurofilament heavy polypeptide1 aa 1-200aa
Immunogen Range	690-740/1026
Clonality	Monoclonal
Clone #	4C3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4744
Swiss Prot	P12036
Synonyms	Neurofilament heavy polypeptide, 200 kDa neurofilament protein, Neurofilament triplet H protein, NF-H, NEFH
Background	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC

Applications

Reactivity

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