NF-H Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52851r-cy7-100uL |
| Product Name | NF-H Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | Human Neurofilament heavy polypeptide1 aa 1-200aa |
| Immunogen Range | 690-740/1026 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4744 |
| Swiss Prot | P12036 |
| Synonyms | Neurofilament heavy polypeptide, 200 kDa neurofilament protein, Neurofilament triplet H protein, NF-H, NEFH |
| Background | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. |
| Application Dilution | |
| WB | IFIHC-P1:100-500 |
| IF(IHC-P) | WB1:500-2000 |
Frequently asked questions
Which applications is NF-H Recombinant Antibody, Cy7 Conjugated (bsm-52851R-Cy7) validated for, and at what dilution?
bsm-52851R-Cy7 is validated for WB(IF(IHC-P)) (1:100-500); IF(IHC-P)(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NF-H Recombinant Antibody, Cy7 Conjugated (bsm-52851R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52851R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NF-H Recombinant Antibody, Cy7 Conjugated (bsm-52851R-Cy7)?
Human Neurofilament heavy polypeptide1 aa 1-200aa Immunogen range: 690-740/1026.
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-52851R-Cy7)?
Cy7 on bsm-52851R-Cy7: excitation / emission 743nm/767nm.