ATPB (16C6) Monoclonal Antibody

$Lane 1: Hela cells lysates; Lane 2: HepG2 cells lysates; Line 3: 293T cells lysates; Probed with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela cells lysates; Lane 2: HepG2 cells lysates; Line 3: 293T cells lysates; Probed with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:200 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:200 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with ATPB (16C6) Monoclonal Antibody (bsm-52904R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with ATPB (16C6) Monoclonal Antibody (bsm-52903R) at 1:200 in HUVEC cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with ATPB (16C6) Monoclonal Antibody (bsm-52903R) at 1:200 in HUVEC cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-52904R
Product Name	ATPB (16C6) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human ATPB between 100 to 250 amino aids
Clonality	Monoclonal
Clone #	16C6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	506
Swiss Prot	P06576
Synonyms	ATP synthase subunit beta, mitochondrial, ATP5B
Background	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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