ATPB Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52904r-bf594-100uL |
| Product Name | ATPB Recombinant Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | Human ATPB between 100 to 250 amino aids |
| Clonality | Monoclonal |
| Clone # | 16C6 |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 506 |
| Swiss Prot | P06576 |
| Synonyms | ATP synthase subunit beta, mitochondrial, ATP5B |
| Background | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
| Application Dilution | |
| WB | 1:1000-2000 |
| IF(IHC-P) | IFIHC-P1:200-500 |
| IF(ICC) | IFICC1:100-200 |
Frequently asked questions
Which applications is ATPB Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-52904R-BF594) validated for, and at what dilution?
bsm-52904R-BF594 is validated for WB(WB) (1:1000-2000); IF(IHC-P)(IF(IHC-P)) (1:200-500); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATPB Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-52904R-BF594) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52904R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATPB Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-52904R-BF594)?
Human ATPB between 100 to 250 amino aids
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-52904R-BF594)?
AbBy Fluor-594 on bsm-52904R-BF594: excitation / emission 590nm/617nm.