VAMP2 Recombinant Antibody

Flow cytometric analysis of sh-sy5y cells with VAMP2 (5C4) Monoclonal Antibody (bsm-52925R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)

$Lane 1: HUVEC cells lysates; Lane 2: Human placenta cells lysates; Probed with VAMP2 (5C4) Monoclonal Antibody (bsm-52925R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HUVEC cells lysates; Lane 2: Human placenta cells lysates; Probed with VAMP2 (5C4) Monoclonal Antibody (bsm-52925R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with VAMP2 (5C4) Monoclonal Antibody (bsm-52925R) at 1:100 in N2A cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with VAMP2 (5C4) Monoclonal Antibody (bsm-52925R) at 1:100 in N2A cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with VAMP2 (5C4) Monoclonal Antibody (bsm-52925R) at 1:100 in SHG-44 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with VAMP2 (5C4) Monoclonal Antibody (bsm-52925R) at 1:100 in SHG-44 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with VAMP2 (5C4) Monoclonal Antibody (bsm-52925R) at 1:100 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with VAMP2 (5C4) Monoclonal Antibody (bsm-52925R) at 1:100 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bsm-52925r
Product Name	VAMP2 Recombinant Antibody
Applications	WB, FCM, IHC-P, IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human VAMP2 between 1-100 amino acids
Immunogen Range	1-50/116
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6844
Swiss Prot	P63027
Synonyms	Vesicle-associated membrane protein 2, Synaptobrevin-2, VAMP-2, VAMP2
Background	Involved in the targeting and/or fusion of transport vesicles to their target membrane. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200