NEFM Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52932r-cy5-100uL |
| Product Name | NEFM Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human NEFM |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4741 |
| Swiss Prot | P07197 |
| Subcellular location | Cytoplasm |
| Synonyms | Neurofilament medium polypeptide; 160 kDa neurofilament protein; neurofilament 3; Nefm; 150kDa medium; NEF 3; NEF3; NEFM; Neurofilament 3; Neurofilament medium polypeptide; Neurofilament protein medium; Neurofilament triplet M protein; Neurofilament3; NF M; NF160; Neurofilament-M; Neurofilament M; NFM; NFM_HUMAN. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:100-200 |
Frequently asked questions
Which applications is NEFM Recombinant Antibody, Cy5 Conjugated (bsm-52932R-Cy5) validated for, and at what dilution?
bsm-52932R-Cy5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NEFM Recombinant Antibody, Cy5 Conjugated (bsm-52932R-Cy5) react with?
Tested reactivity: Human.
How should bsm-52932R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NEFM Recombinant Antibody, Cy5 Conjugated (bsm-52932R-Cy5)?
A synthesized peptide derived from human NEFM
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-52932R-Cy5)?
Cy5 on bsm-52932R-Cy5: excitation / emission 625,650nm/670nm.