A2M Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52944r-100uL |
| Product Name | A2M Monoclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | A synthesized peptide derived from human alpha 2 Macroglobulin |
| Clonality | Recombinant |
| Clone # | 1C6 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2 |
| Swiss Prot | P01023 |
| Subcellular location | Cytoplasm, Secreted |
| Synonyms | A2MD; CPAMD5; FWP007; S863-7; A2mp; A2MAC1; A2m1; A2maa; A2mb; Mam; A2MG_HUMAN; A2M; Alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; PZP_MOUSE; Pzp; Alpha-2-macroglobulin (Alpha-2-M); A2MG_RAT; alpha-2-macroglobulin |
| Background | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. |
| Application Dilution | |
| WB | 1:1000-2000 |
| IHC-P | IHC-P1:100-500 |
| IHC-F | IHC-F1:100-500 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is A2M Monoclonal Antibody (bsm-52944R) validated for, and at what dilution?
bsm-52944R is validated for WB(WB) (1:1000-2000); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does A2M Monoclonal Antibody (bsm-52944R) react with?
Tested reactivity: Human.
How should bsm-52944R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise A2M Monoclonal Antibody (bsm-52944R)?
A synthesized peptide derived from human alpha 2 Macroglobulin