TDP43 (6C6) Monoclonal Antibody

$Lane 1: Hela cells lysates; Lane 2: K563 cells lysates; Probed with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n\r\n$

Lane 1: Hela cells lysates; Lane 2: K563 cells lysates; Probed with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n\r\n

Flow cytometric analysis of HeLa cells with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$IF(ICC) staining with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:200 in SH-SY5Y cells (red) . The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:200 in SH-SY5Y cells (red) . The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-52949R
Product Name	TDP43 (6C6) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human TDP43 between 250-350 amino acids
Clonality	Monoclonal
Clone #	6C6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	23435
Swiss Prot	Q13148
Synonyms	DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, POLR2A
Background	DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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