TDP43 Monoclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52949r-100uL |
| Product Name | TDP43 Monoclonal Antibody |
| Applications |
WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Zebrafish |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | A synthesized peptide derived from human TDP43 |
| Clonality | Recombinant |
| Clone # | 6C6 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 23435 |
| Swiss Prot | Q13148 |
| Subcellular location | Nucleus |
| Synonyms | ALS10; TDP-43; 1190002A23Rik; Tdp43; TADBP_HUMAN; TARDBP; TADBP_MOUSE; TAR DNA binding protein |
| Background | DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. |
| Application Dilution | |
| WB | 1:1000-5000 |
| FCM | FCM1:50-100 |
| IHC-P | IHC-P1:100-500 |
| IHC-F | IHC-F1:100-500 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is TDP43 Monoclonal Antibody (bsm-52949R) validated for, and at what dilution?
bsm-52949R is validated for WB(WB) (1:1000-5000); FCM(FCM) (1:50-100); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TDP43 Monoclonal Antibody (bsm-52949R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Zebrafish.
How should bsm-52949R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TDP43 Monoclonal Antibody (bsm-52949R)?
A synthesized peptide derived from human TDP43