FGFR2 Recombinant Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52953r-100uL |
| Product Name | FGFR2 Recombinant Antibody |
| Applications |
WB
IP
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Extracellular domain of Human FGFR2 |
| Clonality | Monoclonal |
| Clone # | 12C7 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 2263 |
| Swiss Prot | P21802 |
| Synonyms | Fibroblast growth factor receptor 2, K-sam, KeRatinocyte growth factor receptor, CD332, FGFR-2, KGFR, FGFR2 |
| Background | Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. |
| Application Dilution | |
| WB | =1:500-2000 |
| IP | IP=1:10-50 |
Frequently asked questions
Which applications is FGFR2 Recombinant Antibody (bsm-52953R) validated for, and at what dilution?
bsm-52953R is validated for WB (WB=1:500-2000); IP (IP=1:10-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-52953R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FGFR2 Recombinant Antibody (bsm-52953R)?
Extracellular domain of Human FGFR2