RbAp 46/48 Monoclonal Antibody

$Western blot was performed using nuclear extracts from HeLa cells (20 \u03bcg) and RbAp 46\/48 Monoclonal Antibody (bsm-53000M) diluted 1:1,000 in TBS-Tween containing 5% skim milk, followed by secondary antibody incubation and exposure.$

Western blot was performed using nuclear extracts from HeLa cells (20 \u03bcg) and RbAp 46\/48 Monoclonal Antibody (bsm-53000M) diluted 1:1,000 in TBS-Tween containing 5% skim milk, followed by secondary antibody incubation and exposure.

Applications

WB
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-53000M
Product Name	RbAp 46/48 Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastoma-binding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG2b
Concentration	Not Determined
Purification	Ascites fluid from mouse containing 0.05% azide
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	5928
Swiss Prot	Q09028
Synonyms	Histone-binding protein RBBP4, RBBP4, p48, RBBP-4, RBAP48
Background	Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200