H3pan (1B2) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-53008M-50ug |
| Product Name | H3pan (1B2) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Others |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Monoclonal antibody raised in mouse against histone H3, using a KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein. |
| Clonality | Monoclonal |
| Clone # | 1B2 |
| Isotype | IgG3 |
| Concentration | 0.5ug/ul |
| Purification | Protein A purified monoclonal antibody in PBS containing 0.05% azide. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 8350 |
| Swiss Prot | P68431 |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is H3pan (1B2) Monoclonal Antibody (bsm-53008M) validated for, and at what dilution?
bsm-53008M is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does H3pan (1B2) Monoclonal Antibody (bsm-53008M) react with?
Tested reactivity: Human, Others.
How should bsm-53008M be stored?
Store at -20C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise H3pan (1B2) Monoclonal Antibody (bsm-53008M)?
Monoclonal antibody raised in mouse against histone H3, using a KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein.