H3K27me2/3 (11D1) Monoclonal Antibody

$A Dot Blot analysis was performed to test the cross reactivity of the H3K27me2\/3 (11D1) Monoclonal Antibody (bsm-53009M) with peptides containing other modifications of histone H3. Other modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of lysines 9 and 36. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:300.$

A Dot Blot analysis was performed to test the cross reactivity of the H3K27me2\/3 (11D1) Monoclonal Antibody (bsm-53009M) with peptides containing other modifications of histone H3. Other modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of lysines 9 and 36. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:300.

Applications

WB
dot-blot

Reactivity

Human

Overview
Catalog #	bsm-53009M
Product Name	H3K27me2/3 (11D1) Monoclonal Antibody
Applications	WB, dot-blot
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Monoclonal antibody raised in mouse against histone H3, di- or trimethylated at lysine 27 (H3K27me2/3), using a KLH-conjugated synthetic peptide.
Clonality	Monoclonal
Clone #	11D1
Isotype	Not determined
Concentration	1.5ug/ul
Purification	Protein G purified monoclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300
Storage Buffer	PBS with 0.05% sodium azide and 0.05% Proclin300.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	8350
Swiss Prot	P68431
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
WB	1:300-5000
dot-blot

Applications

Reactivity

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