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H3K27me2/3 (11D1) Monoclonal Antibody

A Dot Blot analysis was performed to test the cross reactivity of the H3K27me2\/3 (11D1) Monoclonal Antibody (bsm-53009M) with peptides containing other modifications of histone H3. Other modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of lysines 9 and 36. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:300.
A Dot Blot analysis was performed to test the cross reactivity of the H3K27me2\/3 (11D1) Monoclonal Antibody (bsm-53009M) with peptides containing other modifications of histone H3. Other modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of lysines 9 and 36. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:300.
Bioss unconjugated antibody vial

Applications

  • WB
  • dot-blot

Reactivity

  • Human
Overview
Catalog # bsm-53009M
Product Name H3K27me2/3 (11D1) Monoclonal Antibody
Applications
WB DOT-BLOT
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Monoclonal antibody raised in mouse against histone H3, di- or trimethylated at lysine 27 (H3K27me2/3), using a KLH-conjugated synthetic peptide.
Clonality Monoclonal
Clone # 11D1
Isotype Not determined
Concentration 1.5ug/ul
Purification Protein G purified monoclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 8350
Swiss Prot P68431
Background Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
WB 1:300-5000
dot-blot

Frequently asked questions

Which applications is H3K27me2/3 (11D1) Monoclonal Antibody (bsm-53009M) validated for, and at what dilution?

bsm-53009M is validated for WB (1:300-5000); dot-blot. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does H3K27me2/3 (11D1) Monoclonal Antibody (bsm-53009M) react with?

Tested reactivity: Human.

How should bsm-53009M be stored?

Store at -20C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise H3K27me2/3 (11D1) Monoclonal Antibody (bsm-53009M)?

Monoclonal antibody raised in mouse against histone H3, di- or trimethylated at lysine 27 (H3K27me2/3), using a KLH-conjugated synthetic peptide.

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