H3K27ac (1A3) Monoclonal Antibody

Applications

  • WB
  • ELISA
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-53022M
Product Name H3K27ac (1A3) Monoclonal Antibody
Applications WB, ELISA, IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Monoclonal antibody raised in mouse against histone H3 acetylated at lysine 27 (H3K27ac), using a KLHconjugated synthetic peptide.
Clonality Monoclonal
Clone # 1A3
Isotype IgG1
Concentration 1ug/ul
Purification Protein A purified monoclonal antibody in PBS containing 0.05% azide.
Storage Buffer PBS with 0.05% sodium azide.
Storage Condition Store at -20°C for 12 months.
Target
Background Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called histone code. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IF(ICC) 1:50-200