Interleukin-4 (3F4) Monoclonal Antibody

$IF(ICC) staining with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100 in D3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100 in D3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Ramos, 2: Raji, 3: Daudi, 4: MOLT-4, 5: EL4.IL-2 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 17 kDa Observed MW: 14 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Ramos, 2: Raji, 3: Daudi, 4: MOLT-4, 5: EL4.IL-2 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 17 kDa Observed MW: 14 kDa

$Lane 1: Recombinant human IL-4 protein, His (HEK293) (bs-43501P) probed with IL-4 Monoclonal Antibody, Unconjugated (bsm-54002R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant human IL-4 protein, His (HEK293) (bs-43501P) probed with IL-4 Monoclonal Antibody, Unconjugated (bsm-54002R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54002R
Product Name	Interleukin-4 (3F4) Monoclonal Antibody
Applications	IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	3565
Clonality	Monoclonal
Clone #	3F4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P05112
Subcellular location	Secreted
Synonyms	Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; IL-4; BSF-1; IL4
Background	Interleukin-4 (IL-4), also designated B cell stimulatory factor-1, is a glycosylated cytokine secreted by activated T lymphocytes, basophils and mast cells. The secreted IL-4 protein promotes the growth and differentiation of cells that participate in immune defense by favoring such events as the expansion of the Th2 lineage relative to Th1 cells. These T helper cell subsets are defined by their pattern of cytokine secretion: Th1 cells secrete IL-2, TNF_ and IFN-_, while Th2 cells secrete IL-4, IL-5 and IL-10. Another key immunological function of IL-4 is to induce immunoglobulin class switching. IL-4 has been shown to induce the production of IgE and enhance IgG4 secretion by B cells, but suppress the production of IgM, IgA, IgG1, IgG2, and IgG3. It has been determined that Stat6 is rapidly tyrosine phosphorylated following stimulation of IL-3 or IL-4, but is not detectably phosphorylated following stimulation with IL-2, IL-12 or erythropoietin.
Application Dilution
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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