ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.", "Category"=>"Primary Antibody", "Clonality"=>"Monoclonal", "Clone Number"=>"3G4", "Concentration"=>"1ug/ul", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human, Mouse", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bsm-54004R", "Gene ID"=>"2956", "Gene ID Link"=>"Entrez Gene:
2956", "Host"=>"Rabbit", "images"=>"[{\"id\":\"24722\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"\",\"product_id\":\"16927\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"1:100\",\"cross_reactive_species_id\":\"2\",\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with MSH6 (3G4) Monoclonal Antibody (bsm-54004R) at 1:100, overnight at 4\\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":null,\"sample_tissue_id\":\"6\",\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"16927\\/24722_raw.jpg\",\"filepath_med\":\"16927\\/24722_med.jpg\",\"filepath_thumb\":\"16927\\/24722_thumb.jpg\",\"display_order\":\"2\",\"lock_version\":\"2\",\"created_at\":\"2019-02-26 01:11:03\",\"updated_at\":\"2019-03-25 16:21:50\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/24722\"},{\"id\":\"24723\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"\",\"product_id\":\"16927\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"1:100\",\"cross_reactive_species_id\":\"2\",\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with MSH6 (3G4) Monoclonal Antibody (bsm-54004R) at 1:100, overnight at 4\\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":null,\"sample_tissue_id\":\"11\",\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"16927\\/24723_raw.jpg\",\"filepath_med\":\"16927\\/24723_med.jpg\",\"filepath_thumb\":\"16927\\/24723_thumb.jpg\",\"display_order\":\"3\",\"lock_version\":\"2\",\"created_at\":\"2019-02-26 01:13:40\",\"updated_at\":\"2019-03-25 16:22:04\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/24723\"},{\"id\":\"24724\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"\",\"product_id\":\"16927\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"1:100\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with MSH6 (3G4) Monoclonal Antibody (bsm-54004R) at 1:100, overnight at 4\\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":null,\"sample_tissue_id\":\"22\",\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"16927\\/24724_raw.jpg\",\"filepath_med\":\"16927\\/24724_med.jpg\",\"filepath_thumb\":\"16927\\/24724_thumb.jpg\",\"display_order\":\"4\",\"lock_version\":\"2\",\"created_at\":\"2019-02-26 01:14:56\",\"updated_at\":\"2019-03-25 16:21:40\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/24724\"},{\"id\":\"28332\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"\",\"product_id\":\"16927\",\"productconjugation_id\":null,\"application_id\":\"1\",\"application_note\":\"\",\"dilution\":\"1:500\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Lane 1: Hela Cells; Probed with MSH6 (3G4) Monoclonal Antibody (bsm-54004R) at 1:500, overnight at 4\\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\\u00b0C.\",\"sample_type_id\":\"1\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":\"13\",\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"16927\\/28332_raw.jpg\",\"filepath_med\":\"16927\\/28332_med.jpg\",\"filepath_thumb\":\"16927\\/28332_thumb.jpg\",\"display_order\":\"5\",\"lock_version\":\"1\",\"created_at\":\"2019-03-25 16:26:32\",\"updated_at\":\"2019-03-25 16:26:32\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/28332\"}]", "Isotype"=>"IgG", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Unmodified", "Notes"=>"Huabio SKU: ET1704-30", "Product URL"=>"https://www.biossusa.com/products/bsm-54004R", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"C terminal Human MSH6 ", "Storage"=>"Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.", "Storage Buffer"=>"Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.", "Storage Condition"=>"Store at -20°C for 12 months.", "Subcellular Locations"=>"Nucleus", "Swiss Prot"=>"P52701", "Swiss Prot Link"=>"Swiss Prot:
P52701", "Synonyms"=>"DNA mismatch repair protein Msh6; G/T mismatch-binding protein; MutS protein homolog 6; MutS-alpha 160 kDa subunit; hMSH6; GTBP; GTMBP; p160; MSH6", "Target Protein"=>"MSH6"} -->
| Overview |
| bsm-54004R |
| MSH6 (3G4) Monoclonal Antibody |
| WB, IHC-P, IF(ICC) |
| Human, Mouse |
| Specifications |
| Unconjugated |
| Rabbit |
| C terminal Human MSH6 |
| Monoclonal |
| 3G4 |
| IgG |
| 1ug/ul |
| Purified by Protein A. |
| Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. |
| Store at -20°C for 12 months. |
| Target |
| 2956 |
| P52701 |
| Nucleus |
| DNA mismatch repair protein Msh6; G/T mismatch-binding protein; MutS protein homolog 6; MutS-alpha 160 kDa subunit; hMSH6; GTBP; GTMBP; p160; MSH6 |
| Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. |
| Application Dilution |
| WB |
1:300-5000 |
| IHC-P |
1:200-400 |
| IF(ICC) |
1:50-200 |
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