GATA1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54013r-cy3 |
| Product Name | GATA1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Human GATA1 between 1-200 amino acids |
| Immunogen Range | 1-250/413 |
| Clonality | Monoclonal |
| Clone # | 8C3 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2623 |
| Swiss Prot | P15976 |
| Synonyms | Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1, GATA1, ERYF1, GF1 |
| Background | Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is GATA1 Recombinant Antibody, Cy3 Conjugated (bsm-54013R-Cy3) validated for, and at what dilution?
bsm-54013R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GATA1 Recombinant Antibody, Cy3 Conjugated (bsm-54013R-Cy3) react with?
Tested reactivity: Human.
How should bsm-54013R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GATA1 Recombinant Antibody, Cy3 Conjugated (bsm-54013R-Cy3)?
Human GATA1 between 1-200 amino acids Immunogen range: 1-250/413.
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-54013R-Cy3)?
Cy3 on bsm-54013R-Cy3: excitation / emission 512,550nm/570,615nm.