LXR alpha Recombinant Antibody, PE-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54023r-pe-cy7-100uL |
| Product Name | LXR alpha Recombinant Antibody, PE-Cy7 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | C terminal Human LXR alpha |
| Immunogen Range | 60-110/447 |
| Clonality | Monoclonal |
| Clone # | 4A8 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10062 |
| Swiss Prot | Q13133 |
| Subcellular location | Nucleus |
| Synonyms | Oxysterols receptor LXR-alpha; LXR alpha; Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3; NR1H3; LXRA; RLD-1. |
| Background | Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is LXR alpha Recombinant Antibody, PE-Cy7 Conjugated (bsm-54023R-PE-Cy7) validated for, and at what dilution?
bsm-54023R-PE-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LXR alpha Recombinant Antibody, PE-Cy7 Conjugated (bsm-54023R-PE-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54023R-PE-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LXR alpha Recombinant Antibody, PE-Cy7 Conjugated (bsm-54023R-PE-Cy7)?
C terminal Human LXR alpha Immunogen range: 60-110/447.
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bsm-54023R-PE-Cy7)?
PE-Cy7 on bsm-54023R-PE-Cy7: excitation / emission 488nm/780nm.