CD3E Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54036m-cy3-100ul |
| Product Name | CD3E Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from mouse CD3E |
| Clonality | Monoclonal |
| Clone # | 4F8 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12501 |
| Swiss Prot | P22646 |
| Subcellular location | Cell membrane |
| Synonyms | CD3epsilon; IMD18; T3E; TCRE; CD3; CD3E_HUMAN; CD3E; T-cell surface antigen T3/Leu-4 epsilon chain; CD3E_MOUSE; CD3 epsilon subunit of T-cell receptor complex; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e molecule; T-cell surface glycoprotein CD3 epsilon chain |
| Application Dilution | |
| WB | 1:1000-2000 |
| IF | 1:200-800 |
Frequently asked questions
Which applications is CD3E Monoclonal Antibody, Cy3 Conjugated (bsm-54036M-Cy3) validated for, and at what dilution?
bsm-54036M-Cy3 is validated for WB (1:1000-2000); IF (1:200-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD3E Monoclonal Antibody, Cy3 Conjugated (bsm-54036M-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54036M-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD3E Monoclonal Antibody, Cy3 Conjugated (bsm-54036M-Cy3)?
KLH conjugated synthetic peptide derived from mouse CD3E
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-54036M-Cy3)?
Cy3 on bsm-54036M-Cy3: excitation / emission 512,550nm/570,615nm.